Saint Augustine and Friedrich Schleiermacher Research Paper

Saint Augustine and Friedrich Schleiermacher - Research Paper Example This paper will provide biographical examination of Saint Augustine as well as Friedrich Schleiermacher, comparing and contrasting their legacy, analyzing their significance in general and to Christianity in particular. The first prominent figure that will be discussed in this paper is Saint Augustine. This Christian saint was born in the middle of fourth century in the territory of the modern Algeria1. It must be noted that his mother is recognized as a saint, namely Saint Monica, but his father remained to be a pagan until his death, when he finally decided to be baptized. Augustine had a wonderful education, especially in rhetoric which is considered to be the strong part of his individuality; despite this, he was never fluent in Greek, one of the major languages of the time. During his youth, he experienced all the pleasures of life, not being very pious. He also adopted various worldviews, which he later claimed to be false. At the age of thirty he was baptized by his close frie nd Ambrose of Milan and since they became a significant figure in the Christian world. The next individual whose legacy will be examined is Friedrich Schleiermacher. He was born many centuries after Saint Augustine and in a completely different cultural background. He was born in the middle of eighteenth century in Prussia into a family of a Christian pastor2. Quite early Schleiermacher adopted love for religion and was genuinely interested in pursuing this path. However, he always rejected blind faith into dogmas and rebelled against orthodoxy.

DNA Restriction and Electrophoresis Lab Report Example | Topics and Well Written Essays - 1000 words

DNA Restriction and Electrophoresis - Lab Report Example The various endonucleases used areECORI, Bain HI, and Hind III.Each of these enzymes has at least five or more restriction sites on the chromosome and it consequently produces six or more restriction fragments of different lengths. The DNA strands are then immersed in a well of 0.8% agarose gel, which is the chosen medium.An electrical field, is applied to the gel evenly which makes the DNA fragments move faster from their original placement towards the positively charged electrode. The smaller DNA fragments travel faster through the gel than the larger ones. The gel actually acts like a sieve. Depending on the restriction enzyme used the DNA fragments separate into distinct bands during electrophoresis. The bands are then stained with a dye to make the invidual restriction enzymes evidently distinguishing them with a dye that attaches itself to the DNA molecule. Electrophoresing can be detected by hydrogen gas bubbling from the negative electrode and oxygen being given off by the positive electrode. These are products of the electrolysis of water. This is the first sign that current is flowing through electrophoresis system. Shortly afterwards bands of loading dye should be seen moving into the gel and migrating towards the positive pole of the apparatus. ... The movement of the DNA fragments are highlighted by the dye which forms coloured bands.(See above and below) Results: Depending on the endonuclease used, the digested DNA samples, exhibit the characteristic bands produced by each restriction. The enzyme is seen because of a dye that is introduced and shows the pattern of movement of the DNA fragments. Various restriction enzymes: There are several types of restriction enzymes, each of which react differently with the DNA and create different cuts. there are some that cut a three base pair sequence while others can cut four six or even eight. Each enzyme has different properties that are factors that determine the efficiency with which the DNA is cut and under what conditions optimum results will be obtained. Manufacturers of these restriction enzymes usually provide a specific buffer solution that is unique to the enzyme. This buffer solution is a mix of cat ions and other components that optimize the efficiency of the enzyme for cutting. Different restriction enzymes have different optimal temperatures under which they function. Conclusion: It can be observed that each sample of purified DNA reacts differently with the varied restriction enzymes. The fourth sample used for the negative control remains intact; as it has been incubated without, an endonuclease.The new DNA fragment can then be extracted from the gel by cutting it and doing gel purification. Vector ligation is used as the insert point. The ligation of the vector has to be cut also by a restriction